Table 4.
Nuclear segregation failure and growth of wild-type, cnm67Δ single, and double mutants
| Strain | Sum of bi- and multinucleate cells (%) | Increase in bi- and multinucleate cells relative to cnm67Δ (%) | Growth of cnm67Δ double mutants relative to cnm67Δ |
|---|---|---|---|
| Wild type | 0.8 | ||
| cnm67Δ | 33.0 | Slow growth | |
| Microtubule motors | |||
| dhc1Δ | 9 | ||
| dhc1Δ cnm67Δ | 30.9 | −6.4 | Unchanged |
| kip3Δ | 3.0 | ||
| kip3Δ cnm67Δ | 39.5 | +19.7 | Much reduced |
| kar3Δ | 3.0 | ||
| kar3Δ cnm67Δ | 41.0 | +24.2 | Much reduced |
| kip2Δ | 5.0 | ||
| kip2Δ cnm67Δ | 25.0 | −24.2 | Improved |
| Microtubule-cortex interaction mediators | |||
| kar9Δ | 6.0 | ||
| kar9Δ cnm67Δ | 37.5 | +13.6 | Reduced |
| num1Δ | 4.0 | ||
| num1Δ cnm67Δ | 25.5 | −22.7 | Improved |
The number of Hhf2-GFP–labeled nuclei was determined in haploid mutant strains in exponential growth phase. More than 800 cells were counted for each strain. For comparison of growth the corresponding single and double mutants were streaked on the same plate containing solid full medium. The plates were incubated at 30 and 20°C for 72 h. cnm67Δ cells showed a clear slow growth phenotype; all other single mutants showed only slightly reduced growth compared with wild-type.