Table 5.
Dynamics of chromatin separation and spindle elongation in diploid wild-type and cnm67Δ cells
| Formation of
bar-shaped nucleus (Hhf2-GFP label)
|
Formation of
hourglass nucleus (Hhf2-GFP label)
|
Spindle
elongation (GFP-Tub1 label)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Maximal length (μm) | Time required (min) | No. of cells measured | Maximal length (μm) | Total time required (min) | No. of cells measured | Maximal length (μm) | Total time required (min) | No. of cells measured | |
| Wild-type | 5.5 (ς = 0.17) | 4.2 (ς = 0.5) | 30 | 7.9 (ς = 0.2) | 11 (ς = 2.1) | 30 | 9.47 (ς = 0.54) | 23.8 (ς = 2.4) | 50 |
| cnm67Δ | 5.4 (ς = 0.19) | 3.8 (ς = 0.4) | 30 | 8.1 (ς = 0.2) | 10 (ς = 2.7) | 30 | 9.56 (ς = 0.56) | 26.2 (ς = 2.8) | 50 |
The average fluorescent diameter of the round-shaped Hhf2-GFP–labeled nuclei was 2.5 μm (ς = 0.16; n = 50); the average-length of the short GFP-Tub 1 labeled spindle prior to elongation was 1.9 μm (ς = 0.14; n = 50). The last frame of a time-lapse sequence showing a round-shaped nucleus or unelongated spindle before anaphase was taken as time 0 min.