Figure 2.

Lowering pkl1 copy number exacerbates the gtb1-PL301 phenotype. Phase microscopy of wild-type cells (972 h⁻) or SL1 cells with pkl1⁺ on a multicopy plasmid (SL1/pSMPkl1) is compared with SL1 cells with only the native copy of pkl1⁺ present (gtb1-PL301) grown at either 30°C (first three panels) or 35°C (last panel). Bent and branched cells were observed only when the gene for Pkl1p was not present in multicopy. Bar, 5 μm.