. Author manuscript; available in PMC: 2006 Jun 30.

Published in final edited form as: J Chromatogr A. 2004 May 7;1035(2):211–219. doi: 10.1016/j.chroma.2004.02.068

Table 5.

Comparison of the separation factors of allo- and threo-configurated diasteromeric amino acids. Separation was considered insufficient when a value of 1.000 ± 0.009 was achieved.

	L-allo/L-threo	L-allo/D-threo	L-allo/D-allo	L-threo/D-threo	L-threo/D-allo	D-allo/D-threo
FDAA
Thr	0.990	0.774	0.861	0.781	0.869	0.899
N-MeThr	1.210	1.118	0.955	0.924	0.789	1.171
Ile	1.004	0.863	0.861	0.860	0.858	1.003
N-MeIle	1.011	0.964	0.926	0.953	0.916	1.041
β-MeOTyr¹	0.966	1.006	0.963	1.041	0.997	1.045
β-MeOTyr¹	1.126	1.130	1.079	1.004	0.958	1.048
GITC
Thr	0.977	0.903	0.971	0.924	0.994	0.930
N-MeThr	1.049	0.962	1.006	0.917	0.959	0.957
Ile	0.997	0.945	0.947	0.948	0.950	0.998
N-MeIle	1.011	0.981	0.944	0.971	0.934	1.040
β-MeOTyr¹	1.057	1.028	1.041	0.973	0.985	0.988
S-NIFE
Thr	0.996	0.918	0.958	0.921	0.962	0.958
N-MeThr	1.081	0.989	0.976	0.915	0.902	1.014
Ile	1.003	0.899	0.899	0.897	0.897	1.000
N-MeIle	0.861	0.820	0.818	0.952	0.950	1.002
β-MeOTyr¹	1.114	0.992	0.916	0.890	0.822	1.083
OPA-IBLC
Thr	1.053	1.033	0.971	0.981	0.923	1.060
Ile	1.006	0.973	0.973	0.968	0.968	1.000
β-MeOTyr¹	0.940	0.988	0.934	1.051	0.993	1.059

The configuration L-allo refers to L-erythro in case of β-MeOTyr.