Figure 3.

ARF binds to the N-terminal, most highly conserved region of GGAs. A variety of deletion constructs of all three GGA ORFs were tested in two-hybrid assays when paired with [Q71L]ARF3, as described in MATERIALS AND METHODS. The three domains of GGAs, shown in Figure 2B, are indicated, along with the specific GGA fragments that were cloned into the two-hybrid expression vector and the results of β-galactosidase assays. The minimal predicted fragment that includes the ARF-binding domain, determined by consensus from all three GGAs, is shown at the bottom.