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Fluorescence spectroscopy of binding of the Specifier 2AP98 with the valine codon to ASLGlyGCC. Fluorescence emission spectra were recorded for the Val Specifier 2AP98 alone (blue diamond), reconstitution of the 5′-UTR Stem I complex between Val Specifier 2AP98 and Common (pink square), the binding of ASLValUACto the Val Specifier 2AP98 and Common complex (green diamond), and the nonbinding control of ASLArgCCG (yellow triangle).
