FIGURE 8.

Close-up view of the interacting site between A1067 and the D-loop of the tRNA. (A) Cryo-EM map of the 70S ribosome (30S: yellow, 50S: blue) bound with the EF-Tu·aa-tRNA·GDP·kirromycin ternary complex (red). The binding region is indicated by a box. (B) Enlarged view of the cryo-EM map in the binding region. The asterisk indicates a density region previously unexplained. (C) Stereo-view showing the fitting of MDI into the map. In this conformation, A1067 has a close contact with the D-loop of the aa-tRNA. (D) Stereo-view showing MDII fitted into the cryo-EM map. In C and D, the cryo-EM map is rendered semitransparent for a better view of the fitting positions. The atomic structure of the Glu62-loop is detailed in the lower panel.