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Intracellular localization of transfected Cy3-labeled siRNA. Transfection with Lipofectamine (D–F), Stearyl-R8 (G–I), and AVP (J–L) results in a lysosomal localization of intracellular Cy3-labeled siRNA, as demonstrated by colocalization with the lysosomal marker Lamp1 24 h after transfection. Nonconjugated siRNA (A–C) shows a less-intense fluorescence pattern, indicating low-transfection efficiency.
