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. 2006 Jul;12(7):1431–1438. doi: 10.1261/rna.2252206

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FIGURE 3. — Neuronal uptake of Cy3-labeled siRNA transfected with Stearyl-R8 shown by time-lapse microscopy (8 h). (Left) Start of live imaging; (right) end of live imaging in phase contrast; (middle) sequential images of live microscopy taken in intervals of 60 min. Arrows indicate the same neuron throughout the time-lapse imaging. SiRNA-containing, Cy3-positive vesicles in the culture medium adhere to the cell membrane. Cellular Cy3 fluorescence representing transfected siRNA increases over time in the cell cytosol, sparing the nucleus.