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RNAi induction in primary neuronal cultures. Lipofectamine (B), Stearyl-R8 (C), and AVP (D) effectively induce siRNA-mediated gene silencing in primary neurons previously transfected with an EGFP-expressing plasmid. EGFP-fluorescence (A–D), phase contrast (E–H) microscopy, and quantification of EGFP-positive cells (I) are shown.
