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Design of splicing reporter and export reporter plasmids. The numbers indicate the nucleotides of AMA1 (nucleotides 900–1320) fused to CUP1 and mark the first nucleotide of the intron (nucleotide 1184) and the first nucleotide of exon 2 (nucleotide 1277). The octagonal stop sign indicates the location of premature stop codons in the constructs. Analogous plasmids were constructed for MER2 and MER3 and included the entire exon 1, intron, and ∼25 nt of exon 2.
