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. 2006 Jul;12(7):1361–1372. doi: 10.1261/rna.2276806

FIGURE 5.

FIGURE 5.

Growth and export assays for (A) yeast containing the AMA1-CUP1 export reporter and MER1 alleles that do not activate splicing, including the activation domain (MER1 AD) or KH domain (MER1 KH) fragments; (B) nam8Δ yeast carrying the AMA1-CUP1 export reporter; and (C) yeast carrying the AMA1-CUP1 export reporter with a G1A mutation in the intron, which abolishes its splicing. Media contain 150 μM copper, and cells were grown for 3 d at 30°C. (D) Primer extension assay for splicing of RNAs from cells containing various export reporters and MER1 alleles described in the text. Splicing efficiencies are reported below each lane using the formula S/(S + U) × 100. (E) Growth of yeast with and without Mer1p on 100 μM copper for strains containing the AMA1 export reporter or export reporter variant containing mutations to the enhancer element (ME), which abolish Mer1p-activated splicing.