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. 2006 Jul 4;4(8):e242. doi: 10.1371/journal.pbio.0040242

Figure 1. A 71-kDa Protein Co-Purifies with FLAG-Nipped-B by Anti-FLAG Affinity Chromatography.

Figure 1

Nuclear extracts of y w; P[ Chip-FLAG-Nipped-B, w+] (FLAG) embryos and y w control embryos were bound to anti-FLAG beads, washed, and eluted with FLAG peptide as described in the text. FLAG-Nipped-B fusion protein was detected in the y w; P[ Chip-FLAG-Nipped-B, w+] eluate, but not in the y w eluate, by anti-FLAG Western blot (top panel). Other eluted proteins were detected by silver stain (bottom panel). A 71-kDa protein specific to the FLAG-Nipped-B eluate was identified by mass spectrometry as the product of the Drosophila CG4203 gene. It is closely related to the human MAU-2 protein. Higher molecular weight bands specific to the FLAG-Nipped-B extract contain multiple proteins whose identities could not be established unambiguously. In the bottom panel, the p71 protein and several other proteins, including the markers, became doublets when the gel was dried for photography. All appeared as single bands before drying.