Figure 3.

Truncation analysis of the PC6B-cd by immunofluorescence. AtT-20 cells expressing either fur/f-PC6B or one of the PC6B-cd truncation constructs (Δ2, Δ5, or Δ8; see Figure 1A) were incubated with mAb M1 (6 μg/ml) for 10 min and fixed either directly (A–D) or after an additional 50-min chase (E–H). The fixed cells in panels E–H were then stained with mAb M2 (I–L). Alternatively (M–P), cells were treated with 10 μg/ml BFA for 20 min before fixation and staining with mAb M1. The primary mAbs were distinguished with the use of subtype-specific secondary antisera.