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. Author manuscript; available in PMC: 2007 Mar 24.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Jan 24;341(4):958–963. doi: 10.1016/j.bbrc.2006.01.048

Figure 1.

Figure 1

Sensitivity and linearity of heparanase ELISA method. A. Anti-heparanase 1E1 monoclonal antibody preferentially recognizes the 8+50 active heparanase heterodimer. Microtiter plate was coated with 1E1 monoclonal antibody. Latent 65 kDa heparanase (◆) and active 8+50 kDa heparanase (■) were added at the indicated amounts, and the ELISA procedure was carried out as described in “Materials and Methods”. Note that the 1E1 antibody preferentially recognizes the 8+50 kDa heterodimer. B. Dose response. Sensitivity of the heparanase ELISA method was evaluated by applying the indicated amounts (pg/well) of the 8+50 kDa active heparanase and plotting the O.D. results on a semi logarithmic scale.