. Author manuscript; available in PMC: 2006 Jul 4.

Published in final edited form as: J Am Soc Nephrol. 2005 Apr 20;16(6):1645–1653. doi: 10.1681/ASN.2004121060

Table 5.

Real-time PCR of bone marrow stromal cells from WT and mutant mice

Gene	WT	Hyp	Mepe^−/−	Mepe^−/−/Hyp	P
Phex	0.01 ± 0.001^a	Not detectable	0.09 ± 0.017^b	Not detectable	0.0003
Akp2	0.35 ± 0.043^a	0.39 ± 0.115^a	1.87 ± 0.593^b	0.28 ± 0.073^a	0.0165
Cathepsin B	4.89 ± 0.613^a	3.60 ± 1.043^a	3.15 ± 0.622^a	3.20 ± 0.281^a	0.3232
Mepe	0.002 ± 0.0005^a	0.008 ± 0.0028^b	Not detectable	Not detectable	0.0035
Dmp1	0.13 ± 0.028^a	0.17 ± 0.023^a	0.32 ± 0.055^b	0.12 ± 0.017^a	0.0120
Osteocalcin	0.05 ± 0.012^a	0.06 ± 0.012^a	0.53 ± 0.067^b	0.06 ± 0.009^a	<0.0001
Osteoprotegerin	0.04 ± 0.004^a	0.04 ± 0.006^a	0.02 ± 0.003^a	0.03 ± 0.005^a	0.1174
Fgf23	0.0006 ± 0.0001^a	0.0046 ± 0.0012^b	0.0001 ± 0.00001^a	0.0064 ± 0.0020^b	0.0022
sfrp4	0.11 ± 0.003^a	0.08 ± 0.010^a	0.09 ± 0.007^a	0.08 ± 0.013^a	0.0920
RankL	0.001 ± 0.00025^a	0.001 ± 0.00017^a	0.0001 ± 0.00002^b	0.0012 ± 0.00025^a	0.0147
Runx2 II	0.011 ± 0.0004^a	0.007 ± 0.0012^b	0.017 ± 0.0014^c	0.008 ± 0.0007^ab	0.0004
Osterix	0.004 ± 0.0007^a	0.003 ± 0.0005^a	0.014 ± 0.0037^b	0.004 ± 0.0002^a	0.0109

Data shown are mean ± SEM from bone marrow stromal cell cultures from WT and mutant mice at 14 d of culture duration with ascorbic acid and β-glycerophosphate (n ≥ 3). Values sharing the same letter superscript within each row are not significantly different at P < 0.05 by one-way ANOVA analysis.