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. Author manuscript; available in PMC: 2006 Jul 4.
Published in final edited form as: Vaccine. 2004 Mar 29;22(11-12):1537–1544. doi: 10.1016/j.vaccine.2003.10.013

Fig. 2.

Fig. 2

Effect of Bcl-XL co-expression in vitro: (A) The biological effect of Bcl-XL expression on cells co-transfected with a conventional plasmid (pCMV-EGFP) or replicase-based plasmid (pSin-EGFP) was measured by counting transfected cells using a fluorescence microscope daily for 1 week. Quadruplicate wells were counted in a blinded fashion, and the experiment was repeated with similar results. Data are shown in percent (with SE) based on the cell count 24 h after transfection, which was set to 100%. (B) Bcl-XL expression is not suppressed by co-transfection of cells with a replicase-based plasmid: BHK-21 cells were transfected with pcDNA-Bcl-XL and either a conventional DNA plasmid (pCMV-EGFP) or replicase-based plasmid (pSin-EGFP). To determine whether co-delivery with another plasmid in vivo could affect Bcl-XL expression, Bcl-XL was detected by immunostaining of cell lysates after PAGE and Western blotting. The experiment was repeated yielding the same result.