Table 1.

The terminal phenotype of limB null cells expressing each construct

Construct	Intact LIM domains	Comments	Terminal phenotype when expressed in limB null cells
1	1–5, no N terminus	Deletes non-LIM N terminus	Full complementation; wild-type development
2	1–4	Deletes non-LIM N terminus and C terminus	Mounds dissociate and reaggregate, eventually forming few slug-like structures
3	2–4	Contains only LIM domains 2–4	No complementation; mound arrest
4	2–5	Deletes N terminus and LIM domain 1	Mounds dissociate and reaggregate, eventually forming few slug-like structures
5	4 and 5	Deletes N terminus and LIM domains 1–3	No complementation; mound arrest
6	None	Deletes LIM domains 1–5 and C terminus	No complementation; mound arrest
7	1 and 2	Deletes LIM domains 3–5 and C terminus	No complementation; mound arrest
8	3–5	Deletes LIM domains 1 and 2	Partial complementation; forms abnormal culminant-like structures
9	1–5	Deletes C terminus	Full complementation; wild-type development
C287S,C290S	1 and 3–5	Contains mutation in LIM domain 2	Partial complementation; forms abnormal slug-like structures
C412S,C415S	1–3 and 5	Contains mutation in LIM domain 4	Partial complementation; forms abnormal slug-like structures

The structure of each construct is as follows: 1) residues 190–550 (end of LIM2); 2) residues 190–436; 3) residues 255–436; 4) residues 255–550; 5) residues 366–550; 6) residues 1–210; 7) residues 1–357; 8) residues 1–210, 273–550; 9) residues 1–518. All N-terminal deletions were expressed in-frame from the Act15 promoter with an ATG translation initiation codon.