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Physical association between Brn1p and Smc2p. Total protein extracts from cells expressing HA-Brn1p (from GAL1 promoter on a CEN plasmid) and Myc-Smc2p (from endogenous promoter on a 2-μm plasmid) were immunoprecipitated with anti-Myc antibody (9E10) and analyzed by immunoblotting with anti-HA and anti-Myc antibodies, as indicated. WCE, Whole cell extract (input), 20 μl; Myc-IP, immunoprecipitation from 400 μl of WCE.
