Figure 6.

Overexpression of PAG3, but not its GAP-inactive mutant, inhibits paxillin recruitment to focal contacts. COS-7 cells were transiently transfected by the calcium precipitation method with plasmids encoding EGFP-tagged PAG3 (a and b) or the CA mutant (c and d). Cells were then fixed, and EGFP-PAG3 (a and c) and endogenous paxillin (b and d) were visualized as in Figures 4 and 3, respectively. Focuses were adjusted 0.5 μm above the surface of the glass chamber plate to visualize the focal contacts. The right column is of the same field as the left column, and arrowheads indicate cells expressing EGFP-PAG3 proteins. Bar, 20 μm.