Effects of a loss of KHC on the eye surface. Mitotic recombination was used to generate Khc20/Khc20 null imaginal cells in first instar larvae that were Khc20/Khc+ (test class) or Khc20/Khc+; P{mini-w+, Khc+}/+ (control class). (A, C, and E) Clone in a test class eye (white facets). (B, D, and F) Clone in a control class eye (pale orange facets). (A and B) Light micrographs that show the positions of the clones. No roughening of the eye surface was detected in any clones at this level of resolution. (C–F) Scanning electron micrographs of the same eyes in the same orientations at low (C and D) and at high (E and F) magnification. Note that the test clone shows slight roughness attributable to misalignment of facets and bristles, whereas the control clone does not.