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. 2000 Apr;11(4):1369–1383. doi: 10.1091/mbc.11.4.1369

Figure 3.

Figure 3

iLIF-induced apoptosis is inhibited by CrmA but not Bcl-2 expression. Cos-1 cells cotransfected with LIF-D or LIF-T expression vectors, at a 1:3 ratio with expression vectors for Bcl-2 (pRSV-hBcl-2), CrmA (pCXN2-CrmA), or the control vector pXMT2, were stained 3 d after transfection for LIF protein (LIF) and DNA. Cells were transfected with pmLIF-DX (LIF-D) and pXMT2 (A), pmLIF-TX (LIF-T) and pXMT2 (B), pmLIF-TX and pCXN2-CrmA (C), and pmLIF-TX and pRSV-hBcl-2 (D). Arrows indicate the nuclei of selected LIF-staining cells. Bar, 20 μm. (E) Graphical representation of relative numbers of β-galactosidase-positive cells 3 d after cotransfection of Cos-1 cells with a 1:3 ratio of pmLIF-DXIres (LIF-D) or pmLIF-TXIres (LIF-T) and pXMT2 (control), pRSV-hBcl-2, or pCXN2-CrmA. The number of staining cells in pmLIF-TX transfections is normalized to the corresponding transfections with pmLIF-DX. Means and SDs were calculated from two experiments.