Figure 3.

The presence of its tail limits the expression level of recombinant TgM-D. Western blot analysis used the anti-myc antibody on parasites expressing recombinant mycTgM-A and mycTgM-AΔtail (A) or mycTgM-D and mycTgM-DΔtail (B). The respective expression levels of each full-length and truncated myosin were judged by comparing the signals resulting from the loading of 105, 106, or 107 parasites, respectively. MycTgM-A and mycTgM-AΔtail,as well as mycTgM-DΔtail, were expressed at comparable levels, whereas full-length mycTgM-D was expressed at an approximately 10-fold reduced level.