Figure 7.

The tails of TgM-A and TgM-D are sufficient to confer to GFP both peripheral localization and association with the particulate fraction. (A) Alignment of three highly related apicomplexan myosin tails. The basic residues are boxed. (B) Localization of GFP (a), GFP·TgM-Atail (b), GFP·TgM-Dtail (c), and GFP·PfM-Atail (d). Fluorescence was observed directly (a and b) or after indirect immunofluorescence with anti-myc antibodies (c and d). (C) Recombinant parasites expressing GFP, GFP·TgM-Atail, GFP·TgM-Dtail, and GFP·PfM-Atail were lysed in PBS and separated by high-speed centrifugations in soluble (S) and particulate (P) fraction. The partitioning of GFP and the tail chimeras reflected their respective intracellular localization observed by imunofluorescence microscopy.