Figure 9.

A dibasic motif in the tail of TgM-A is essential for the cell membrane localization of GFP-tail chimera. (A) The wild-type sequence of the tail of TgM-A (wt) is shown aligned with the site-specific mutants (I–III) and the two deletion mutants (Δ14 and Δ22). The mutated residues are indicated in red. (B) Confocal microscopic analysis of parasites stably transformed with the respective mutant tail constructs fused to GFP. Indirect immunofluorescence was performed using the anti-myc antibodies. (C) Anti-myc immunoblot analysis of recombinant parasites expressing the different constructs presented in A.