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. 2000 Apr;11(4):1401–1419. doi: 10.1091/mbc.11.4.1401

Figure 6.

Figure 6

Molecular identification of emb-30. (A) Genetic and physical map of the emb-30III region. (Top) Sequenced cosmids. Sequence tag site analysis (panels with black background) positioned the tnDf2 left breakpoint between C38C10.4 and C48B4.9. (Bottom) Enlarged physical map. ORFs are shown above and below the line, with their directions of transcription being left to right and right to left, respectively. The sod-4 ORF is not shown but would be to the right. Predicted genes from cosmid T26G10 are not deleted, whereas B0464.5 is deleted. The tnDf2 left breakpoint was detected by Southern hybridization with the use of a T26G10.4 probe. A novel 14-kb band is detected in tnDf2/+ heterozygotes (wild-type band, 7.2 kb; ns, nonspecific band). (B) Identification of emb-30 with the use of RNAi. DAPI-stained embryos resulting from injection of F54C8.3 double-stranded RNA (exons 2–15) arrest at the one-cell stage and do not form polar bodies (emb-30 mutant phenocopy). (C) emb-30 is expressed in the germ line and the soma. Northern hybridization to young-adult-stage poly(A)+ RNA from germline developmental mutants: glp-4(bn2ts), no germ line; fem-2(b245ts), oocytes only; and fem-3(q20 gf, ts), sperm only.