Table 5.
Physical and genetic mapping of emb-30
| Parental genotype | Recombinant phenotype | Genotype of recombinant chromosomea | Number |
|---|---|---|---|
| sma-2(e502) emb-30(tn377ts) ced-7(n1892) unc-69(e587)/sod-4(pk68∷Tc1)b | Sma non-Unc | sma-2(e502) sod-4(pk68∷Tc1) | 21 /77 |
| sma-2(e502) emb-30(tn377ts) sod-4(pk68∷Tc1) | 5 /77 | ||
| sma-2(e502) emb-30(tn377ts) | 3 /77 | ||
| sma-2(e502) emb-30(tn377ts) ced-7(n1892) | 48 /77 | ||
| Non-Sma Unc | emb-30(tn377ts) ced-7(n1892) unc-69(e587) | 38 /124 | |
| ced-7(n1892) unc-69(e587) | 3 /124 | ||
| sod-4(pk68∷Tc1) ced-7(n1892) unc-69(e587) | 3 /124 | ||
| sod-4(pk68∷Tc1) unc-69(e587) | 80 /124 | ||
| emb-9(hc70 sd, ts) unc-69(e587)/unc-36(e251) emb-30(tn377ts)c | Non-Emb-9 Unc-69 | unc-36(e251) unc-69(e587) | 4 /82 |
| unc-36(e251) emb-30(tn377ts) unc-69(e587) | 78 /82 | ||
| emb-9(hc70 sd, ts) unc-69(e587)/emb-30(tn471)d | Non-Emb-9 Unc-69 | unc-69(e587) | 3 /55 |
| emb-30(tn471) unc-69(e587) | 52 /55 |
a
Genes are listed in left-to-right order on LGIII. unc-36, sma-2, and unc-69 are not shown in Figure 6.
b
The presence of sod-4(pk68∷Tc1) was scored by PCR (see MATERIALS AND METHODS).
c
Recombinants were picked at 25°C and placed at 15°C.
d
Recombinants were picked at 22.5°C.