Figure 1.

Sequence analysis of Er-1mem. The sequence of Er-1mem that is unique and forms its intracellular domain is printed in italics to distinguish this region from the other region, which is equivalent to Er-1 precursor (i.e., prepro-Er-1) and forms the transmembrane and extracellular domains of Er-1mem. Within prepro-Er-1, arrowheads delimit pre-, pro-, and Er-1. Arrows and relative denominations localize segments identified for synthesizing oligonucleotides used, as forward and reverse PCR primers (right and left orientation, respectively), for producing recombinant proteins p3/10 and p143 of Er-1mem. Residues in bold identify the potentially immunogenic segment, as revealed by the program PROTEAN (DNASTAR, Madison, WI), taken as reference to generate anti-1p25/39 antibodies.