Figure 5.

Dose- and cation-dependent analysis of the effect of ADAM 23 on NB100 adhesion. (A) Culture dishes were coated with the indicated concentrations of ADAM 23, BSA, and GST and blocked for nonspecific binding with BSA (2.5%). Cells were allowed to adhere for 2 h at 37°C. Nonbound cells were removed by washing, and adhesion was determined by counting bound cells per unit area using a 20× high-power objective and an ocular grid. (B) Analysis of the effect of divalent cations on NB100 adhesion mediated by ADAM 23. The experiments were performed as above with the exception that cells were washed three times in PBS and resuspended in the same buffer supplemented either with 1 mM MgCl₂, 50 μM MnCl₂, and 1 mM CaCl₂ or 1 mM MgCl₂ plus 5 mM EDTA. C, control cells, corresponding to NB100 cells plated on wells coated with BSA in the absence of divalent cations. The results presented are the mean of three independent experiments.