Figure 9.

Delineation of the ADAM 23 region mediating the interaction with αvβ3. (A) Effect of a point mutation (E→A) in the sequence of ADAM 23 on its adhesive-promoting properties represented as percentage of adhesion using neuroblastoma cell lines. ADAM 23 wild-type and experiments using mutant ADAM 23 are shown as white and black bars, respectively. Inset, SDS-PAGE gel with both ADAM 23 wild-type (lane 1) and E→A mutant (lane 2). M, molecular weight markers. (B) Effect of a synthetic peptide (AVNECDIT, pep 330) on the adhesion of NB100 cells. Pep 331 indicates the assay carried out with a scrambled peptide (DCVTNIAE). Two different concentrations of peptides were used: 20 μg/ml (black bars) and 40 μg/ml (gray bars). White bars indicate control using NB100 cells grown on ADAM 23.