Figure 6.

Northern blot analyses. (A) Linearized map of wild-type mtDNA. The COX II/Cyt b fusion gene and the predicted size of its transcript, are shown below the map, as are the in and out probes (bold) used to detect the authentic and fusion mRNAs. The four tRNAs examined in this study, and the direction of their transcription (leftward, L-strand encoded; rightward, H-strand encoded), are also shown. (B) Northern blots of representative cybrid lines (notation as in Figure 2) showing transcription in the breakpoint region, using the in and out probes. The expected sizes of the indicated transcripts are shown at right.