Figure 1.

Neurite outgrowth in PC12 and N18 cells in response to TS. (A–D) PC12 cells, mechanically stripped of their neurites, were seeded in serum-free RPMI medium on collagen-coated plastic dishes with no addition (A), 50 ng/ml NGF (B), or 100 ng/ml TS (C). Pictures were taken 24 h after addition of the reagents. Magnification, 400×. (D) Quantitation of PC12 cells exhibiting neurite outgrowth (triplicate samples) from an experiment similar to that shown in A–C. (E) Neurite outgrowth of N18 neuroblastoma cells by TS and rTS-F. Microtiter wells were coated overnight in triplicate with laminin (Ln), TS, rTS clones 7F and 19Y, and BSA and used as substratum for attachment of N18 cells. Cells exhibiting neurite extension were counted 17 h later. The values are the mean ± SEM of three separate experiments.