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. 2000 Apr;11(4):1487–1498. doi: 10.1091/mbc.11.4.1487

Figure 4.

Figure 4

Figure 4

Identification of a TS domain that promotes neuroprotection. (A) Diagram of the linear structure of rTS and truncated derivatives obtained by PCR technology (see MATERIALS AND METHODS). Numbers under the TS diagram refer to amino acid positions in clone 19Y (GenBank accession number AJ002174). The biological activities indicated on the right are TS activity, neurite extension (NE), and antiapoptose (a-AP). These activities were measured using purified polypeptides (see MATERIALS AND METHODS). + indicates that the polypeptide promoted significant NE or a-AP in a dose-dependent manner, as in B below. Otherwise, the polypeptide was considered inactive (−). (B) Inhibition of apoptosis by endogenous and recombinant fragments of TS. PC12 cells were grown in serum-free RPMI in the presence of the indicated concentrations of TS and rTS-F, isolated from trypomastigotes and E. coli, respectively.