Figure 8.

Induction of Bcl-2 Expression in PC12 cells. Bcl-2 expression in total RNA was isolated 17 h after addition of the growth factors and was assessed by RT-PCR using primers specific for Bcl-2 and for control GAPDH. Molecular weight markers (in base pairs) are indicated. (A) Bcl-2 expression in PC12 cells cultured in serum-free medium without (RPMI) and with NGF (100 ng/ml) or TS (100 and 200 ng). (B) Bcl-2 expression induced by TS (2.5 ng/ml) plus CNTF (50 ng/ml). Bcl-2 transcripts were not detected when TS (2.5 ng/ml) and CNTF (50 ng/ml) were added separately to PC12 cells in serum-free medium (RPMI).