Figure 2.

Decorin and biglycan production by FBCs. Immunoprecipitation with the use of antibodies specific for decorin and biglycan from sulfate-labeled cells demonstrates that FBCs secrete decorin but not biglycan. Shown is an autoradiogram of immunoprecipitated products. Arrows indicate the gel positions of the intact proteoglycan and the core protein after chondroitinase ABC digestion. [³⁵S]cysteine and [³⁵S]sulfate were used to radiolabel the core protein and GAG side chains, respectively. Molecular mass markers are in kilodaltons.