Figure 7.

Association of CLIC5 with cytoskeletal components in the affinity chromatography assay. A soluble extract of placental microvilli (lanes 1) was incubated with glutathione–agarose affinity beads containing GST (lanes 2), GST-ezrin 475–584 (lanes 3), and GST-ezrin 475–586 (lanes 4). The beads were washed and eluted, and the bound proteins were run on 12% SDS-PAGE and blotted. (A) Blot probed with antibody against CLIC5. (B) Blot probed with antibody against CLIC4. (C) Blot probed with antibody against α-actinin.