Figure 6.

Response of Xenopus egg extracts to an oligonucleotide duplex and M13 DNA in the presence and absence of Xcds1. (A) Depletion of Xcds1 does not diminish the mitotic delay caused by an oligonucleotide duplex. Sperm nuclei (500 nuclei/μl) (●, ▪) or both sperm nuclei (500 nuclei/μl) and oligonucleotide duplex (oligo 1 + oligo 2) (50 ng/μl) (○, □) was added to Xcds1-depleted (○, ●) or mock-depleted (□, ▪) extracts. Sperm nuclei (500 nuclei/μl) were also added to untreated extracts (▴). The timing of nuclear envelope breakdown (NEB) was monitored by microscopy. (B) Same as A except that the response to M13 DNA (10 ng/μl) was examined.