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Coomassie blue-stained SDS-PAGE of freshly formed filaments prepared from sucrose gradient fractions containing essentially pure proteins A2 and B1 (lane 1) and excised and aggressively reduced 68- and 70-kDa bands (lanes 3 and 4, respectively). Lane 2 is a sample of 40S hnRNP particles included as an internal marker and to show the proteins typically present in isolated 40S monoparticles.
