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Stable expression of LckY505F and LckΔSH2 requires Hsp90. NIH-3T3 cells transfected with wt Lck (A), LckY505F (B), or LckΔSH2 (C) were treated without (−) or with 5 μM geldanamycin (GA), 10 μM radicicol (Rad), or 100 μg/ml cycloheximide (Chx) for 3.5 h. Cell lysates, containing equal amounts of protein, were immunoblotted with antibodies against Lck (A) or against both Lck and Src (B and C).
