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Hsp90 activity is required only during the synthesis of Lck. SupT1 cells were labeled for 5 min with [35S]methionine/cysteine and chased as indicated. Radicicol (Rad) was either absent (A), added at 10 μM together with [35S]methionine/cysteine at the beginning of the pulse and present during the chase (B), or present during the chase only (C). Lck was immunoprecipitated as indicated in MATERIALS AND METHODS. Lck, coimmunoprecipitated CD4, and the background band (★) are indicated.
