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UD-GFP does not require Hsp90 for synthesis. HeLa cells transfected with UD-GFP (A) or Lck (B) were labeled for 5 min with [35S]methionine/cysteine and chased as indicated in either the absence (−) or presence (+) of 5 μM geldanamycin (GA) as described in MATERIALS AND METHODS. UD-GFP and Lck were immunoprecipitated with anti-Lck serum from detergent lysates.
