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Temperature-sensitive growth and Calcofluor White sensitivity of gpi11:: LEU2-pPIG-F cells. Wild-type (GPI11) and gpi11:: LEU2 strains harboring pPIG-F were streaked on YPD agar and incubated for 4 d at 25 or 37°C (A) or on YPD agar with or without 0.032 mg of Calcofluor White/ml and incubated for 4 d at 30°C (B).
