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Lipids 11-1 and 11-2 are both radiolabeled with [3H]ethanolamine. The ethanolamine-auxotrophic psd1::TRP1/psd2:: HIS3/gpi11::LEU2-pPIG-F strain was suspended in SD medium lacking ethanolamine and choline and radiolabeled at 25 or 37°C with either [3H]ethanolamine (50 μCi) or [3H]inositol. Radiolabeled lipids were extracted, separated by TLC in solvent B, and detected by fluorography. Lipids 11-1 and 11-2 are marked with arrows.
