Figure 5.

Developmental timing of the gdt1 mutant. Ax2 and L8 cells were harvested from axenic medium, washed, and spotted at a density of 1 × 10⁸ cells/ml on phosphate-agar. Development at 22°C was monitored with the microscope. At 7 h, L8 cells had aggregated, whereas Ax2 showed faint ripples; at 13 h some tipped aggregates were observed in Ax2, and L8 started to form fingers. At 15 h, tipped aggregates were found in Ax2, whereas all L8 cells were in the finger stage. At 19 h, L8 was in late culmination, whereas Ax2 was only beginning with culmination.