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. 2000 May;11(5):1673–1685. doi: 10.1091/mbc.11.5.1673

Figure 5.

Figure 5

DNA-binding properties of GST-NP6. (A) The purity of 50 ng of GST-NP6 eluted from a FPLC Mono-S column was determined by PhastGel SDS-PAGE (Pharmacia) and silver staining (lane 2). A Western blot of a similar aliquot of the same Mono-S fraction, with the use of anti-GST antibody, is shown in lane 1. Protein molecular mass markers are in lane 3. (B) EMSA of GST-NP6 with 10 fmol of ARS1 as the probe. Buffer only (lane 1) and GST only (1000 ng, lane 2) were used as controls. GST-NP6 was added at 5 ng (lane 3), 10 ng (lane 4), and 30 ng (lanes 5–9). For a competition assay, 0.2 μg of poly(dA-dG):poly(dC-dT) (Poly dN; lane 6), 100 ng of the 283-bp ARS1 (lane 7), 30 ng of the 210-bp ARS1-B (lane 8), and 10 ng of the 70-bp ARS1-A (lane 9) were also added.