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. 2000 May;11(5):1673–1685. doi: 10.1091/mbc.11.5.1673

Figure 8.

Figure 8

Figure 8

Growth properties of transformants carrying wild-type CDC6 and mutant cdc6(AG6) genes. (A) cdc6-1 cells were transformed with the mutated plasmid YCp111-cdc6(AG6) carrying the cdc6 DNA-binding mutant. The plasmids YCp111-CDC6 (wild-type gene) and YCp111 (vector only) were used as positive and negative controls, respectively. Yeast transformants carrying the indicated plasmids were grown at SD-leuΔ at room temperature until early log phase. The cultures were then shifted to 37°C (nonpermissive temperature) and harvested at various times, and cells were counted with the use of a hemacytometer. The value given represents an average of three or four independent determinations. (●) YCp111-CDC6; (▴) YCp111-cdc6(AG6); (○) YCp111 only. (B) SD-leu plates were incubated with wild-type CDC6 and cdc6(AG6) mutant–transformed cells. Plates were then incubated at 25 and 37°C, respectively. Cells transformed with vector YCp111 only were used as the negative control. Plates were photographed after 30 h of incubation.