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Subcellular distribution of epitope-tagged ZO-1 mutants in corneal epithelial cells. Monolayers of corneal epithelial cells stably expressing truncated ZO-1 proteins were fixed and immunostained. Antibodies against ZO-1 (B, D, F, H, and J) were used to detect the localization of endogenous ZO-1, whereas antibodies to the myc (A, C, E, and G) and FLAG (I) epitope tags demonstrate the subcellular localization of the stably transfected mutant proteins. Bar, 21 μm.
