Figure 4.

Vph1p degradation was partially dependent on the HRD genes and UBC7. (A) Degradation of Vph1p in a vma21Δ strain. Results of pulse–chase experiment of VMA21 (RHY566) and vma21Δ (RHY918) strains are shown. Cells were pulse-labeled with ³⁵S-Express for 10 min and chased for the indicated times. Vph1p was immunoprecipitated and analyzed by SDS-PAGE and autoradiography. (B) Pulse–chase experiment of strains containing the vma21Δ allele (parent strain: RHY918) and the hrd1Δ allele (RHY1032), the hrd2-1 allele (RHY1067), the hrd3Δ allele (RHY1034), the ubc6Δ allele (RHY1228), the ubc7Δ allele (RHY1069), the ubc6Δubc7Δ allele (RHY1488), or the hrd1Δ ubc7Δ allele (RHY1491). (C) Densitometric analysis of the pulse–chase experiments in B. Each value is the average of at least two independent pulse–chase experiments. SDs were <10%.