Figure 8.

Inhibition of Rho-kinase and PAK alters focal adhesion reorganization in HGF-stimulated MDCK cells. MDCK cells injected with expression vectors (0.2 mg/ml) encoding Myc-tagged RB/PH(TT) (b) or Myc-tagged PAKR (c) were incubated for 2 h and then stimulated with HGF for 4 and 3 h, respectively. Cells were then incubated with Triton X-100–containing CSK buffer and fixed with 3.7% formaldehyde. Vinculin-containing focal complexes/adhesions were revealed with the use of anti-vinculin mAb followed by anti-mouse Cy3. Injected areas are identified by open arrows. Bar, 25 μm.