Table 1.
Cdc42, Rac, PAK, and Rho-kinase are involved in HGF-induced lamellipodia formation in MDCK cells
| cDNA | Percent of cells with lamellipodiaa | SD (%) | pb |
|---|---|---|---|
| PRK5 | 100 | – | – |
| N17Cdc42 | 55 | ±6 | <0.05 |
| N17Rac | 34 | ±15 | <0.05 |
| RB/PH(TT) | 36 | ±6 | <0.05 |
| PAKR | 19 | ±1 | <0.05 |
a
Not all injected cells will form lamellipodia. The percentage of cells injected with the control vector pRK5 that formed lamellipodia was normalized to 100%, and the values obtained in cells expressing the dominant negative mutants were modified accordingly (see MATERIALS AND METHODS).
b
A statistical analysis (analysis of variance) was performed on the results obtained in these experiments and gave p < 0.05, which confirms that they are highly significant.